Construct: shRNA TRCN0000089114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175166.2-1969s1c1
DNA Barcode:
GCAGCGATTATATGACAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5430419D17Rik (71395)
Gene Description:
RIKEN cDNA 5430419D17 gene
Transcript:
RefSeq NM_175166.2 (NON-CURRENT)
Match location:
Position 1969 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCGATTATATGACAACAA
Hairpin Sequence:
5'-CCGG-GCAGCGATTATATGACAACAA-CTCGAG-TTGTTGTCATATAATCGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCGATTATATGACAACAACTCGAGTTGTTGTCATATAATCGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCGATTATATGACAACAACTCGAGTTGTTGTCATATAATCGCTGC-3'

Other clones with same target sequence:

(none)