Construct: shRNA TRCN0000089189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011915.1-344s1c1
DNA Barcode:
GCCAGCCATTCCTGTCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wif1 (24117)
Gene Description:
Wnt inhibitory factor 1
Transcript:
RefSeq NM_011915.1 (NON-CURRENT)
Match location:
Position 344 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCCATTCCTGTCAATAT
Hairpin Sequence:
5'-CCGG-GCCAGCCATTCCTGTCAATAT-CTCGAG-ATATTGACAGGAATGGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCCATTCCTGTCAATATCTCGAGATATTGACAGGAATGGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCCATTCCTGTCAATATCTCGAGATATTGACAGGAATGGCTGGC-3'

Other clones with same target sequence:

(none)