Construct: shRNA TRCN0000089223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008231.2-2003s1c1
DNA Barcode:
CCCACTAAGAATACAGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hdgf (15191)
Gene Description:
hepatoma-derived growth factor
Transcript:
RefSeq NM_008231.2 (NON-CURRENT)
Match location:
Position 2003 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTAAGAATACAGGGAAA
Hairpin Sequence:
5'-CCGG-CCCACTAAGAATACAGGGAAA-CTCGAG-TTTCCCTGTATTCTTAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTAAGAATACAGGGAAACTCGAGTTTCCCTGTATTCTTAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTAAGAATACAGGGAAACTCGAGTTTCCCTGTATTCTTAGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000332744