Construct: shRNA TRCN0000089230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080438.1-649s1c1
DNA Barcode:
CCTCAATTTCTGTTGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glra3 (110304)
Gene Description:
glycine receptor, alpha 3 subunit
Transcript:
RefSeq NM_080438.1 (NON-CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAATTTCTGTTGAAAGAA
Hairpin Sequence:
5'-CCGG-CCTCAATTTCTGTTGAAAGAA-CTCGAG-TTCTTTCAACAGAAATTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAATTTCTGTTGAAAGAACTCGAGTTCTTTCAACAGAAATTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAATTTCTGTTGAAAGAACTCGAGTTCTTTCAACAGAAATTGAGG-3'

Other clones with same target sequence:

(none)