Construct: shRNA TRCN0000089238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486520.1-41s1c1
DNA Barcode:
GCCTTCTACAGCATAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434656 (434656)
Gene Description:
similar to [Mouse murinoglobulin mRNA, complete cds.], gene product
Transcript:
RefSeq XM_486520.1 (NON-CURRENT)
Match location:
Position 41 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTACAGCATAGTATTT
Hairpin Sequence:
5'-CCGG-GCCTTCTACAGCATAGTATTT-CTCGAG-AAATACTATGCTGTAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTACAGCATAGTATTTCTCGAGAAATACTATGCTGTAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTACAGCATAGTATTTCTCGAGAAATACTATGCTGTAGAAGGC-3'

Other clones with same target sequence:

(none)