Construct: shRNA TRCN0000089312
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175644.1-485s1c1
- DNA Barcode:
- CACCACTGAGTGTGAGACCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gphb5 (217674)
- Gene Description:
- glycoprotein hormone beta 5
- Transcript:
- RefSeq NM_175644.1 (NON-CURRENT)
- Match location:
- Position 485 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000089312)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CACCACTGAGTGTGAGACCAT
- Hairpin Sequence:
-
5'-CCGG-CACCACTGAGTGTGAGACCAT-CTCGAG-ATGGTCTCACACTCAGTGGTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCACCACTGAGTGTGAGACCATCTCGAGATGGTCTCACACTCAGTGGTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACACCACTGAGTGTGAGACCATCTCGAGATGGTCTCACACTCAGTGGTG-3'
Other clones with same target sequence:
(none)
