Construct: shRNA TRCN0000089337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010754.2-1156s1c1
DNA Barcode:
CTAAGTGATAGTGCAATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad2 (17126)
Gene Description:
SMAD family member 2
Transcript:
RefSeq NM_010754.2 (NON-CURRENT)
Match location:
Position 1156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGTGATAGTGCAATCTTT
Hairpin Sequence:
5'-CCGG-CTAAGTGATAGTGCAATCTTT-CTCGAG-AAAGATTGCACTATCACTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGTGATAGTGCAATCTTTCTCGAGAAAGATTGCACTATCACTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGTGATAGTGCAATCTTTCTCGAGAAAGATTGCACTATCACTTAG-3'

Other clones with same target sequence:

  1. TRCN0000327372