Construct: shRNA TRCN0000089341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139299.1-1605s1c1
DNA Barcode:
GCCGTATTCAATCCAAGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il31ra (218624)
Gene Description:
interleukin 31 receptor A
Transcript:
RefSeq NM_139299.1 (NON-CURRENT)
Match location:
Position 1605 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTATTCAATCCAAGCTTA
Hairpin Sequence:
5'-CCGG-GCCGTATTCAATCCAAGCTTA-CTCGAG-TAAGCTTGGATTGAATACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTATTCAATCCAAGCTTACTCGAGTAAGCTTGGATTGAATACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTATTCAATCCAAGCTTACTCGAGTAAGCTTGGATTGAATACGGC-3'

Other clones with same target sequence:

(none)