Construct: shRNA TRCN0000089410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008068.1-1509s1c1
DNA Barcode:
CCTTGTGTACTGGATAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra6 (14399)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6
Transcript:
RefSeq NM_008068.1 (NON-CURRENT)
Match location:
Position 1509 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTGTACTGGATAGTTTA
Hairpin Sequence:
5'-CCGG-CCTTGTGTACTGGATAGTTTA-CTCGAG-TAAACTATCCAGTACACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTGTACTGGATAGTTTACTCGAGTAAACTATCCAGTACACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTGTACTGGATAGTTTACTCGAGTAAACTATCCAGTACACAAGG-3'

Other clones with same target sequence:

(none)