Construct: shRNA TRCN0000089468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_125968.1-1363s1c1
DNA Barcode:
GCTATATTTAACACCAGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clhc1 (73324)
Gene Description:
clathrin heavy chain linker domain containing 1
Transcript:
RefSeq XM_125968.1 (NON-CURRENT)
Match location:
Position 1363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATATTTAACACCAGTCAA
Hairpin Sequence:
5'-CCGG-GCTATATTTAACACCAGTCAA-CTCGAG-TTGACTGGTGTTAAATATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATATTTAACACCAGTCAACTCGAGTTGACTGGTGTTAAATATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATATTTAACACCAGTCAACTCGAGTTGACTGGTGTTAAATATAGC-3'

Other clones with same target sequence:

(none)