Construct: shRNA TRCN0000089488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020276.2-2057s1c1
DNA Barcode:
CCTGAAACAATTTGAAGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsmf (56876)
Gene Description:
NMDA receptor synaptonuclear signaling and neuronal migration factor
Transcript:
RefSeq NM_020276.2 (NON-CURRENT)
Match location:
Position 2057 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAACAATTTGAAGGGAA
Hairpin Sequence:
5'-CCGG-CCTGAAACAATTTGAAGGGAA-CTCGAG-TTCCCTTCAAATTGTTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAACAATTTGAAGGGAACTCGAGTTCCCTTCAAATTGTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAACAATTTGAAGGGAACTCGAGTTCCCTTCAAATTGTTTCAGG-3'

Other clones with same target sequence:

(none)