Construct: shRNA TRCN0000089503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010298.3-2373s1c1
DNA Barcode:
CCACCTTAAATCAAAGTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glrb (14658)
Gene Description:
glycine receptor, beta subunit
Transcript:
RefSeq NM_010298.3 (NON-CURRENT)
Match location:
Position 2373 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTTAAATCAAAGTCTAT
Hairpin Sequence:
5'-CCGG-CCACCTTAAATCAAAGTCTAT-CTCGAG-ATAGACTTTGATTTAAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTTAAATCAAAGTCTATCTCGAGATAGACTTTGATTTAAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTTAAATCAAAGTCTATCTCGAGATAGACTTTGATTTAAGGTGG-3'

Other clones with same target sequence:

(none)