Construct: shRNA TRCN0000089524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008542.2-1764s1c1
DNA Barcode:
CCTACTCAACAACCACAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad6 (17130)
Gene Description:
SMAD family member 6
Transcript:
RefSeq NM_008542.2 (NON-CURRENT)
Match location:
Position 1764 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACTCAACAACCACAGATA
Hairpin Sequence:
5'-CCGG-CCTACTCAACAACCACAGATA-CTCGAG-TATCTGTGGTTGTTGAGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACTCAACAACCACAGATACTCGAGTATCTGTGGTTGTTGAGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACTCAACAACCACAGATACTCGAGTATCTGTGGTTGTTGAGTAGG-3'

Other clones with same target sequence:

(none)