Construct: shRNA TRCN0000089555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175419.3-759s1c1
DNA Barcode:
CCTCATCATTTCATCCGGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Actr5 (109275)
Gene Description:
ARP5 actin-related protein 5
Transcript:
RefSeq NM_175419.3 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATCATTTCATCCGGCTA
Hairpin Sequence:
5'-CCGG-CCTCATCATTTCATCCGGCTA-CTCGAG-TAGCCGGATGAAATGATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATCATTTCATCCGGCTACTCGAGTAGCCGGATGAAATGATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATCATTTCATCCGGCTACTCGAGTAGCCGGATGAAATGATGAGG-3'

Other clones with same target sequence:

(none)