Construct: shRNA TRCN0000089576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_110503.6-8836s1c1
DNA Barcode:
CCTGAAAGTAAAGCACCGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Macf1 (11426)
Gene Description:
microtubule-actin crosslinking factor 1
Transcript:
RefSeq XM_110503.6 (NON-CURRENT)
Match location:
Position 8836 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAAGTAAAGCACCGTTA
Hairpin Sequence:
5'-CCGG-CCTGAAAGTAAAGCACCGTTA-CTCGAG-TAACGGTGCTTTACTTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAAGTAAAGCACCGTTACTCGAGTAACGGTGCTTTACTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAAGTAAAGCACCGTTACTCGAGTAACGGTGCTTTACTTTCAGG-3'

Other clones with same target sequence:

(none)