Construct: shRNA TRCN0000089591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484743.1-439s1c1
DNA Barcode:
GACTCTTATGAAAGAGTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433196 (433196)
Gene Description:
similar to tubulin, alpha 1
Transcript:
RefSeq XM_484743.1 (NON-CURRENT)
Match location:
Position 439 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCTTATGAAAGAGTCTAT
Hairpin Sequence:
5'-CCGG-GACTCTTATGAAAGAGTCTAT-CTCGAG-ATAGACTCTTTCATAAGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCTTATGAAAGAGTCTATCTCGAGATAGACTCTTTCATAAGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCTTATGAAAGAGTCTATCTCGAGATAGACTCTTTCATAAGAGTC-3'

Other clones with same target sequence:

(none)