Construct: shRNA TRCN0000089624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010063.1-919s1c1
DNA Barcode:
CGTCAGTTCTACGATGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dync1i1 (13426)
Gene Description:
dynein cytoplasmic 1 intermediate chain 1
Transcript:
RefSeq NM_010063.1 (NON-CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAGTTCTACGATGAACAT
Hairpin Sequence:
5'-CCGG-CGTCAGTTCTACGATGAACAT-CTCGAG-ATGTTCATCGTAGAACTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAGTTCTACGATGAACATCTCGAGATGTTCATCGTAGAACTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAGTTCTACGATGAACATCTCGAGATGTTCATCGTAGAACTGACG-3'

Other clones with same target sequence:

(none)