Construct: shRNA TRCN0000089659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033073.2-827s1c1
DNA Barcode:
CTGGACTTGGATGGTATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krt7 (110310)
Gene Description:
keratin 7
Transcript:
RefSeq NM_033073.2 (NON-CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGACTTGGATGGTATCATT
Hairpin Sequence:
5'-CCGG-CTGGACTTGGATGGTATCATT-CTCGAG-AATGATACCATCCAAGTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGACTTGGATGGTATCATTCTCGAGAATGATACCATCCAAGTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGACTTGGATGGTATCATTCTCGAGAATGATACCATCCAAGTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000324194