Construct: shRNA TRCN0000089682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011569.1-667s1c1
DNA Barcode:
CACTCAACAATAACTCACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tekt1 (21689)
Gene Description:
tektin 1
Transcript:
RefSeq NM_011569.1 (NON-CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCAACAATAACTCACCAA
Hairpin Sequence:
5'-CCGG-CACTCAACAATAACTCACCAA-CTCGAG-TTGGTGAGTTATTGTTGAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCAACAATAACTCACCAACTCGAGTTGGTGAGTTATTGTTGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCAACAATAACTCACCAACTCGAGTTGGTGAGTTATTGTTGAGTG-3'

Other clones with same target sequence:

(none)