Construct: shRNA TRCN0000089716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198711.2-1495s1c1
DNA Barcode:
GCAGGAATTAAGGGAGAACCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col25a1 (77018)
Gene Description:
collagen, type XXV, alpha 1
Transcript:
RefSeq NM_198711.2 (NON-CURRENT)
Match location:
Position 1495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAATTAAGGGAGAACCT
Hairpin Sequence:
5'-CCGG-GCAGGAATTAAGGGAGAACCT-CTCGAG-AGGTTCTCCCTTAATTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAATTAAGGGAGAACCTCTCGAGAGGTTCTCCCTTAATTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAATTAAGGGAGAACCTCTCGAGAGGTTCTCCCTTAATTCCTGC-3'

Other clones with same target sequence:

(none)