Construct: shRNA TRCN0000089754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_207079.4-5214s1c1
DNA Barcode:
CCTGCAAGAATACATGGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sptan1 (20740)
Gene Description:
spectrin alpha, non-erythrocytic 1
Transcript:
RefSeq XM_207079.4 (NON-CURRENT)
Match location:
Position 5214 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAAGAATACATGGCTTT
Hairpin Sequence:
5'-CCGG-CCTGCAAGAATACATGGCTTT-CTCGAG-AAAGCCATGTATTCTTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAAGAATACATGGCTTTCTCGAGAAAGCCATGTATTCTTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAAGAATACATGGCTTTCTCGAGAAAGCCATGTATTCTTGCAGG-3'

Other clones with same target sequence:

(none)