Construct: shRNA TRCN0000089787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_135159.3-1170s1c1
DNA Barcode:
GCTTTCAGTTTATCACCGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG235580 (235580)
Gene Description:
predicted gene, EG235580
Transcript:
RefSeq XM_135159.3 (NON-CURRENT)
Match location:
Position 1170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCAGTTTATCACCGCAA
Hairpin Sequence:
5'-CCGG-GCTTTCAGTTTATCACCGCAA-CTCGAG-TTGCGGTGATAAACTGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCAGTTTATCACCGCAACTCGAGTTGCGGTGATAAACTGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCAGTTTATCACCGCAACTCGAGTTGCGGTGATAAACTGAAAGC-3'

Other clones with same target sequence:

(none)