Construct: shRNA TRCN0000089930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009450.1-232s1c1
DNA Barcode:
GCTGGCAACAAATATGTACCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tubb2a (22151)
Gene Description:
tubulin, beta 2A class IIA
Transcript:
RefSeq NM_009450.1 (NON-CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCAACAAATATGTACCT
Hairpin Sequence:
5'-CCGG-GCTGGCAACAAATATGTACCT-CTCGAG-AGGTACATATTTGTTGCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCAACAAATATGTACCTCTCGAGAGGTACATATTTGTTGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCAACAAATATGTACCTCTCGAGAGGTACATATTTGTTGCCAGC-3'

Other clones with same target sequence:

(none)