Construct: shRNA TRCN0000089936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011835.1-644s1c1
DNA Barcode:
CCAGAGGCAAATAAGTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Katna1 (23924)
Gene Description:
katanin p60 (ATPase-containing) subunit A1
Transcript:
RefSeq NM_011835.1 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGGCAAATAAGTTTGAT
Hairpin Sequence:
5'-CCGG-CCAGAGGCAAATAAGTTTGAT-CTCGAG-ATCAAACTTATTTGCCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGGCAAATAAGTTTGATCTCGAGATCAAACTTATTTGCCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGGCAAATAAGTTTGATCTCGAGATCAAACTTATTTGCCTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000325824