Construct: shRNA TRCN0000089960
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_007871.1-803s1c1
- DNA Barcode:
- GCCCTTGAGAAGAGGCTATAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Dnm2 (13430)
- Gene Description:
- dynamin 2
- Transcript:
- RefSeq NM_007871.1 (NON-CURRENT)
- Match location:
- Position 803 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000089960)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCTTGAGAAGAGGCTATAT
- Hairpin Sequence:
-
5'-CCGG-GCCCTTGAGAAGAGGCTATAT-CTCGAG-ATATAGCCTCTTCTCAAGGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCTTGAGAAGAGGCTATATCTCGAGATATAGCCTCTTCTCAAGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCTTGAGAAGAGGCTATATCTCGAGATATAGCCTCTTCTCAAGGGC-3'
Other clones with same target sequence:
-
TRCN0000317474
