Construct: shRNA TRCN0000089989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027897.2-669s1c1
DNA Barcode:
GCCAGCGTTCTCTTCAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhpn2 (52428)
Gene Description:
rhophilin, Rho GTPase binding protein 2
Transcript:
RefSeq NM_027897.2 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCGTTCTCTTCAACATT
Hairpin Sequence:
5'-CCGG-GCCAGCGTTCTCTTCAACATT-CTCGAG-AATGTTGAAGAGAACGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCGTTCTCTTCAACATTCTCGAGAATGTTGAAGAGAACGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCGTTCTCTTCAACATTCTCGAGAATGTTGAAGAGAACGCTGGC-3'

Other clones with same target sequence:

(none)