Construct: shRNA TRCN0000090003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007733.1-3711s1c1
DNA Barcode:
GCCAACTTCTTTCCATATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col19a1 (12823)
Gene Description:
collagen, type XIX, alpha 1
Transcript:
RefSeq NM_007733.1 (NON-CURRENT)
Match location:
Position 3711 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACTTCTTTCCATATGTT
Hairpin Sequence:
5'-CCGG-GCCAACTTCTTTCCATATGTT-CTCGAG-AACATATGGAAAGAAGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACTTCTTTCCATATGTTCTCGAGAACATATGGAAAGAAGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACTTCTTTCCATATGTTCTCGAGAACATATGGAAAGAAGTTGGC-3'

Other clones with same target sequence:

(none)