Construct: shRNA TRCN0000090034

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007740.2-599s1c1
DNA Barcode:
CCAAACAAAGTCTGTCGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col9a1 (12839)
Gene Description:
collagen, type IX, alpha 1
Transcript:
RefSeq NM_007740.2 (NON-CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090034)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACAAAGTCTGTCGCATT
Hairpin Sequence:
5'-CCGG-CCAAACAAAGTCTGTCGCATT-CTCGAG-AATGCGACAGACTTTGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACAAAGTCTGTCGCATTCTCGAGAATGCGACAGACTTTGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACAAAGTCTGTCGCATTCTCGAGAATGCGACAGACTTTGTTTGG-3'

Other clones with same target sequence:

(none)