Construct: shRNA TRCN0000090043
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_007743.1-329s1c1
- DNA Barcode:
- GCTGCTCAGTATTCTGACAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Col1a2 (12843)
- Gene Description:
- collagen, type I, alpha 2
- Transcript:
- RefSeq NM_007743.1 (NON-CURRENT)
- Match location:
- Position 329 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000090043)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGCTCAGTATTCTGACAAA
- Hairpin Sequence:
-
5'-CCGG-GCTGCTCAGTATTCTGACAAA-CTCGAG-TTTGTCAGAATACTGAGCAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGCTCAGTATTCTGACAAACTCGAGTTTGTCAGAATACTGAGCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGCTCAGTATTCTGACAAACTCGAGTTTGTCAGAATACTGAGCAGC-3'
Other clones with same target sequence:
-
TRCN0000335210
