Construct: shRNA TRCN0000090085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010904.2-3250s1c1
DNA Barcode:
CTTTCCAAAGAGCCTAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nefh (380684)
Gene Description:
neurofilament, heavy polypeptide
Transcript:
RefSeq NM_010904.2 (NON-CURRENT)
Match location:
Position 3250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCCAAAGAGCCTAGCAAA
Hairpin Sequence:
5'-CCGG-CTTTCCAAAGAGCCTAGCAAA-CTCGAG-TTTGCTAGGCTCTTTGGAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCCAAAGAGCCTAGCAAACTCGAGTTTGCTAGGCTCTTTGGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCCAAAGAGCCTAGCAAACTCGAGTTTGCTAGGCTCTTTGGAAAG-3'

Other clones with same target sequence:

(none)