Construct: shRNA TRCN0000090093
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_484375.2-521s1c1
- DNA Barcode:
- CTGTTCCTTGCCCAGTGTGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC432856 (432856)
- Gene Description:
- similar to Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin light chain 3) (MLC-3) (LC17)
- Transcript:
- RefSeq XM_484375.2 (NON-CURRENT)
- Match location:
- Position 521 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000090093)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGTTCCTTGCCCAGTGTGAT
- Hairpin Sequence:
-
5'-CCGG-CTGTTCCTTGCCCAGTGTGAT-CTCGAG-ATCACACTGGGCAAGGAACAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGTTCCTTGCCCAGTGTGATCTCGAGATCACACTGGGCAAGGAACAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGTTCCTTGCCCAGTGTGATCTCGAGATCACACTGGGCAAGGAACAG-3'
Other clones with same target sequence:
(none)
