Construct: shRNA TRCN0000090162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_488371.1-99s1c1
DNA Barcode:
GTTTGATCTGATGTATGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC436224 (436224)
Gene Description:
similar to TuBulin, Alpha (tba-6)
Transcript:
RefSeq XM_488371.1 (NON-CURRENT)
Match location:
Position 99 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGATCTGATGTATGCCAA
Hairpin Sequence:
5'-CCGG-GTTTGATCTGATGTATGCCAA-CTCGAG-TTGGCATACATCAGATCAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGATCTGATGTATGCCAACTCGAGTTGGCATACATCAGATCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGATCTGATGTATGCCAACTCGAGTTGGCATACATCAGATCAAAC-3'

Other clones with same target sequence:

(none)