Construct: shRNA TRCN0000090214

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021895.2-418s1c1
DNA Barcode:
GCACAAGATCAACAATGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Actn4 (60595)
Gene Description:
actinin alpha 4
Transcript:
RefSeq NM_021895.2 (CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090214)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGATCAACAATGTAAA
Hairpin Sequence:
5'-CCGG-GCACAAGATCAACAATGTAAA-CTCGAG-TTTACATTGTTGATCTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGATCAACAATGTAAACTCGAGTTTACATTGTTGATCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGATCAACAATGTAAACTCGAGTTTACATTGTTGATCTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000309727