Construct: shRNA TRCN0000090239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023735.1-472s1c1
DNA Barcode:
GAACCTGAAGATCATTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Actr3 (74117)
Gene Description:
ARP3 actin-related protein 3
Transcript:
RefSeq NM_023735.1 (NON-CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTGAAGATCATTACTTT
Hairpin Sequence:
5'-CCGG-GAACCTGAAGATCATTACTTT-CTCGAG-AAAGTAATGATCTTCAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTGAAGATCATTACTTTCTCGAGAAAGTAATGATCTTCAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTGAAGATCATTACTTTCTCGAGAAAGTAATGATCTTCAGGTTC-3'

Other clones with same target sequence:

  1. TRCN0000287540