Construct: shRNA TRCN0000090273

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146116.1-1391s1c1
DNA Barcode:
GCTGTCTTAGTCACTAAAGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tubb4b (227613)
Gene Description:
tubulin, beta 4B class IVB
Transcript:
RefSeq NM_146116.1 (NON-CURRENT)
Match location:
Position 1391 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090273)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTTAGTCACTAAAGCA
Hairpin Sequence:
5'-CCGG-GCTGTCTTAGTCACTAAAGCA-CTCGAG-TGCTTTAGTGACTAAGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTTAGTCACTAAAGCACTCGAGTGCTTTAGTGACTAAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTTAGTCACTAAAGCACTCGAGTGCTTTAGTGACTAAGACAGC-3'

Other clones with same target sequence:

(none)