Construct: shRNA TRCN0000090315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127139.7-602s1c1
DNA Barcode:
CCAAGTGGATTCGTACAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eml1 (68519)
Gene Description:
echinoderm microtubule associated protein like 1
Transcript:
RefSeq XM_127139.7 (NON-CURRENT)
Match location:
Position 602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGGATTCGTACAGTTT
Hairpin Sequence:
5'-CCGG-CCAAGTGGATTCGTACAGTTT-CTCGAG-AAACTGTACGAATCCACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGGATTCGTACAGTTTCTCGAGAAACTGTACGAATCCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGGATTCGTACAGTTTCTCGAGAAACTGTACGAATCCACTTGG-3'

Other clones with same target sequence:

(none)