Construct: shRNA TRCN0000090339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011586.1-2874s1c1
DNA Barcode:
GCTGAATTACACCAAGCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo18a (360013)
Gene Description:
myosin XVIIIA
Transcript:
RefSeq NM_011586.1 (NON-CURRENT)
Match location:
Position 2874 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATTACACCAAGCAGAA
Hairpin Sequence:
5'-CCGG-GCTGAATTACACCAAGCAGAA-CTCGAG-TTCTGCTTGGTGTAATTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATTACACCAAGCAGAACTCGAGTTCTGCTTGGTGTAATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATTACACCAAGCAGAACTCGAGTTCTGCTTGGTGTAATTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000323828