Construct: shRNA TRCN0000090372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129845.3-5056s1c1
DNA Barcode:
GCCAGTTTCCATCAATTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fn1 (14268)
Gene Description:
fibronectin 1
Transcript:
RefSeq XM_129845.3 (NON-CURRENT)
Match location:
Position 5056 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTTTCCATCAATTATAA
Hairpin Sequence:
5'-CCGG-GCCAGTTTCCATCAATTATAA-CTCGAG-TTATAATTGATGGAAACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTTTCCATCAATTATAACTCGAGTTATAATTGATGGAAACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTTTCCATCAATTATAACTCGAGTTATAATTGATGGAAACTGGC-3'

Other clones with same target sequence:

  1. TRCN0000354173