Construct: shRNA TRCN0000090394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010616.1-1394s1c1
DNA Barcode:
GCAGGAGTTTATGCTGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif12 (16552)
Gene Description:
kinesin family member 12
Transcript:
RefSeq NM_010616.1 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGTTTATGCTGGAGAA
Hairpin Sequence:
5'-CCGG-GCAGGAGTTTATGCTGGAGAA-CTCGAG-TTCTCCAGCATAAACTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGTTTATGCTGGAGAACTCGAGTTCTCCAGCATAAACTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGTTTATGCTGGAGAACTCGAGTTCTCCAGCATAAACTCCTGC-3'

Other clones with same target sequence:

(none)