Construct: shRNA TRCN0000090418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178893.2-2032s1c1
DNA Barcode:
GCCGAAATTCACTGAGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro2a (107684)
Gene Description:
coronin, actin binding protein 2A
Transcript:
RefSeq NM_178893.2 (NON-CURRENT)
Match location:
Position 2032 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAAATTCACTGAGAACAA
Hairpin Sequence:
5'-CCGG-GCCGAAATTCACTGAGAACAA-CTCGAG-TTGTTCTCAGTGAATTTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAAATTCACTGAGAACAACTCGAGTTGTTCTCAGTGAATTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAAATTCACTGAGAACAACTCGAGTTGTTCTCAGTGAATTTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000335626