Construct: shRNA TRCN0000090448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484082.1-178s1c1
DNA Barcode:
GCCTTCCTGAAGAAGAACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432604 (432604)
Gene Description:
similar to keratin intermediate filament 16a
Transcript:
RefSeq XM_484082.1 (NON-CURRENT)
Match location:
Position 178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCCTGAAGAAGAACTAT
Hairpin Sequence:
5'-CCGG-GCCTTCCTGAAGAAGAACTAT-CTCGAG-ATAGTTCTTCTTCAGGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCCTGAAGAAGAACTATCTCGAGATAGTTCTTCTTCAGGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCCTGAAGAAGAACTATCTCGAGATAGTTCTTCTTCAGGAAGGC-3'

Other clones with same target sequence:

(none)