Construct: shRNA TRCN0000090449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484082.1-483s1c1
DNA Barcode:
GCTCAGCATGAAAGCATCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432604 (432604)
Gene Description:
similar to keratin intermediate filament 16a
Transcript:
RefSeq XM_484082.1 (NON-CURRENT)
Match location:
Position 483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGCATGAAAGCATCCTT
Hairpin Sequence:
5'-CCGG-GCTCAGCATGAAAGCATCCTT-CTCGAG-AAGGATGCTTTCATGCTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGCATGAAAGCATCCTTCTCGAGAAGGATGCTTTCATGCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGCATGAAAGCATCCTTCTCGAGAAGGATGCTTTCATGCTGAGC-3'

Other clones with same target sequence:

(none)