Construct: shRNA TRCN0000090455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_126961.5-3249s1c1
DNA Barcode:
CCACTTCCTCTGGTATGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekhh1 (211945)
Gene Description:
pleckstrin homology domain containing, family H (with MyTH4 domain) member 1
Transcript:
RefSeq XM_126961.5 (NON-CURRENT)
Match location:
Position 3249 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCCTCTGGTATGTCAA
Hairpin Sequence:
5'-CCGG-CCACTTCCTCTGGTATGTCAA-CTCGAG-TTGACATACCAGAGGAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCCTCTGGTATGTCAACTCGAGTTGACATACCAGAGGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCCTCTGGTATGTCAACTCGAGTTGACATACCAGAGGAAGTGG-3'

Other clones with same target sequence:

(none)