Construct: shRNA TRCN0000090484
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_139743.6-958s1c1
- DNA Barcode:
- GCCGAATCTTTGGAGAAGTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Afdn (17356)
- Gene Description:
- afadin, adherens junction formation factor
- Transcript:
- RefSeq XM_139743.6 (NON-CURRENT)
- Match location:
- Position 958 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000090484)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCGAATCTTTGGAGAAGTAT
- Hairpin Sequence:
-
5'-CCGG-GCCGAATCTTTGGAGAAGTAT-CTCGAG-ATACTTCTCCAAAGATTCGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCGAATCTTTGGAGAAGTATCTCGAGATACTTCTCCAAAGATTCGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCGAATCTTTGGAGAAGTATCTCGAGATACTTCTCCAAAGATTCGGC-3'
Other clones with same target sequence:
(none)
