Construct: shRNA TRCN0000090499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175484.1-481s1c1
DNA Barcode:
CCCATCACCAAGAATGTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro2b (235431)
Gene Description:
coronin, actin binding protein, 2B
Transcript:
RefSeq NM_175484.1 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCACCAAGAATGTACAT
Hairpin Sequence:
5'-CCGG-CCCATCACCAAGAATGTACAT-CTCGAG-ATGTACATTCTTGGTGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCACCAAGAATGTACATCTCGAGATGTACATTCTTGGTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCACCAAGAATGTACATCTCGAGATGTACATTCTTGGTGATGGG-3'

Other clones with same target sequence:

(none)