Construct: shRNA TRCN0000090538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_282816.4-2251s1c1
DNA Barcode:
GCTCGGAATAATGCTGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5117 (330731)
Gene Description:
predicted gene 5117; trimethylguanosine synthase homolog pseudogene
Transcript:
RefSeq XM_282816.4 (NON-CURRENT)
Match location:
Position 2251 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGGAATAATGCTGAAGTT
Hairpin Sequence:
5'-CCGG-GCTCGGAATAATGCTGAAGTT-CTCGAG-AACTTCAGCATTATTCCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGGAATAATGCTGAAGTTCTCGAGAACTTCAGCATTATTCCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGGAATAATGCTGAAGTTCTCGAGAACTTCAGCATTATTCCGAGC-3'

Other clones with same target sequence:

(none)