Construct: shRNA TRCN0000090595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355324.2-1855s1c1
DNA Barcode:
GCCACCGATGAAGCTTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381358 (381358)
Gene Description:
similar to Spectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain)
Transcript:
RefSeq XM_355324.2 (NON-CURRENT)
Match location:
Position 1855 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCGATGAAGCTTATAAA
Hairpin Sequence:
5'-CCGG-GCCACCGATGAAGCTTATAAA-CTCGAG-TTTATAAGCTTCATCGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCGATGAAGCTTATAAACTCGAGTTTATAAGCTTCATCGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCGATGAAGCTTATAAACTCGAGTTTATAAGCTTCATCGGTGGC-3'

Other clones with same target sequence:

(none)