Construct: shRNA TRCN0000090644

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128002.4-623s1c1
DNA Barcode:
GCCCTGAGTTAAATTCAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cthrc1 (68588)
Gene Description:
collagen triple helix repeat containing 1
Transcript:
RefSeq XM_128002.4 (NON-CURRENT)
Match location:
Position 623 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090644)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGAGTTAAATTCAACTA
Hairpin Sequence:
5'-CCGG-GCCCTGAGTTAAATTCAACTA-CTCGAG-TAGTTGAATTTAACTCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGAGTTAAATTCAACTACTCGAGTAGTTGAATTTAACTCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGAGTTAAATTCAACTACTCGAGTAGTTGAATTTAACTCAGGGC-3'

Other clones with same target sequence:

(none)