Construct: shRNA TRCN0000090704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153078.1-2441s1c1
DNA Barcode:
GCTATCACAAAGCCAAATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehbp1 (216565)
Gene Description:
EH domain binding protein 1
Transcript:
RefSeq NM_153078.1 (NON-CURRENT)
Match location:
Position 2441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCACAAAGCCAAATGTA
Hairpin Sequence:
5'-CCGG-GCTATCACAAAGCCAAATGTA-CTCGAG-TACATTTGGCTTTGTGATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCACAAAGCCAAATGTACTCGAGTACATTTGGCTTTGTGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCACAAAGCCAAATGTACTCGAGTACATTTGGCTTTGTGATAGC-3'

Other clones with same target sequence:

(none)