Construct: shRNA TRCN0000090731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486765.1-581s1c1
DNA Barcode:
GCTATTGCTAGCACCTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434847 (434847)
Gene Description:
similar to 3110007F17Rik protein
Transcript:
RefSeq XM_486765.1 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTGCTAGCACCTTTATT
Hairpin Sequence:
5'-CCGG-GCTATTGCTAGCACCTTTATT-CTCGAG-AATAAAGGTGCTAGCAATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTGCTAGCACCTTTATTCTCGAGAATAAAGGTGCTAGCAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTGCTAGCACCTTTATTCTCGAGAATAAAGGTGCTAGCAATAGC-3'

Other clones with same target sequence:

(none)